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MICROBIOLOGY

Overview

The Department of Microbiology at AMALA IMS aids the patients by putting into use the molecular methods in diagnosis and drug resistance in bacterial, fungal, viral, and parasitic infections, guiding the patients towards total healing.

The Department of Microbiology undertakes Academic activities as well as diagnostic laboratory work. Department of Microbiology is a multidisciplinary unit consisting of Molecular biology, Bacteriology, Immunology (mainly Serological test), Mycology and Mycobacteriology.

The Department is located on the 3rd floor of the Medical College block along with Molecular laboratory in its premises. The central diagnostic laboratory of the Department is located in the ground floor of the Chavara block.

Facilities

  • SERVICE LAB: Diagnostic Facilities
  • Bacteriology - Staining Methods, Gram's, Ziehl-Neelsen, Giemsa, etc. Also includes Culture of samples.
    Urine, Sputum, Secretions, Pus
    Body Fluids like CSF, Ascitic, Pleural, Synovial, etc.
    AFB culture for Pulmonary and Extra Pulmonary Tuberculosis
    Blood Culture - Automated by BacT Alert
    Stock cultures kept

  • Mycology
  • Fungal staining and fungal culture for identification of fungal diseases. Stock cultures kept.

  • Virology
  • Diagnosis of viral diseases like Hepatitis A & E, Dengue, Chikungunya by serological methods.

  • Serology
  • ELISA - for antibody detection of viral diseases and Leptospira antibody,
    Widal,
    RPR & TPHA
    ASO - by Turbidometry.

  • Immunology
  • CRP by Turbidometry
    RA by Turbidometry
    C3 & C4 - by ELISA, ANA profile
    Ab against cyclic citrullinated peptides (ACCP) by ELISA

  • Parasitology
  • Stool Microscopic examination for parasitic ova, cyst and larva
    Blood smear examination for haematological parasites

Services

The department has a dynamic group of Consultant Microbiologists who strive to provide timely and accurate reports to aid in patient care.

Conventional diagnostic modalities have been incorporated along with state of the art automated equipments with latest technologies into the laboratory services.

The Molecular Laboratory is accredited by National Accreditation Board for Testing and Calibration Laboratories (NABL) since August 2020, in COVID-19 RT-PCR (Open and Closed).  Around 20,000 samples in Bacteriology, 1800 samples in Mycology  and 20,000 samples in Serology are reported annually. The department participates in Kerala Antimicrobial Resistance Surveillance Network organised by WHO, to ensure representative AMR data from Amala IMS, updated on a monthly basis using WHONET. The data of notifiable diseases are provided to IDSP.

The Department also plays a major role in the infection control activities of the hospital. Sterility check of blood bags, intravenous fluids and disinfectants are being continuously monitored. Active surveillance cultures for Bone Marrow Transplantation (BMT) units and ICUs and OT are conducted. HICC secretary and medical officer from the department are actively supervising the hospital infection control activities. The faculty also provide monthly training programs to all the hospital staff regarding robust infection control practices.  Infection surveillance data is used to measure success of infection prevention, and control programs and to identify areas of improvement. Continuous surveillance of infections for early detection of outbreak, for which appropriate control measures are undertaken.

The Department of Microbiology participates in the external quality assurance programmes on a regular basis, namely :

  • Bacteriology cultures with Christian Medical College, Vellore.
  •  Serological tests with Christian Medical College, Vellore.

Faculties

Dr Reena John

Professor & HOD

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Dr. Suseela K V

Professor

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Dr. Aiswariya Alex

Associate Professor

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Dr. Subi Das

Associate Professor

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Dr. Geethalakshmi V

Assistant Professor

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Ms. Neethu Babu

Senior Resident

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Dr. Angel Mary George

Senior Resident

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Dr. Renji Francis N

Senior Resident

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Dr. Iswarya Babu P

Senior Resident

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CME

CME Details Date of Conduct
Name : CME : Sepsis Update
Theme/Subject : Sepsis CME Brochure
News Link : CME : Sepsis Update
2023

Publications

  • Suseela KV. Salmonella ser Typhimurium food poisoning – glimpse of clinical problems; J Acad ClinMicrobiol 2003; 5: 81-82.
  • Suseela KV, Santhakumar S, Sathiavathy KA, Rao TV. Salmonella typhimurium Septicemia manifesting as Monoarthritis-case report; J Acad ClinMicrobiol 2007; 9: 89-90
  • Suseela KV, Asok ON, Sathyavathy AK. Mycobacterium fortuitum infection following inguinal hernia repair with mesh: A Case Series. J Acad ClinMicrobiol; 2013;15 :69-71
  • Suseela KV, Criton S, Patil S, Gnagadharan G. A case of Erysipeloid presenting as abscesses along the lymphatics . J Acad ClinMicrobiol; 2015;17 : 45 - 47
  • Suseela KV , Jayaraj V, Betsy T, Ramya M. Incidence of group B streptocooci colonization during the Third Trimester of pregnancy in two tertiary care centers in the central part of Kerala. IJCMR: 2016; 3: 568 - 570
  • Suseela KV, Rennis D, Patil S, Alex A. Bacterial profile and antibiotic susceptibility in chronic obstructive pulmonary disease patients with acute exacerbation. A cross sectional study in a tertiary care hospital. Indian J Microbiol Res 2016; 3 (3):
  • Aiwariya A, Suseela KV , Subi D. Prevalence of Moraxella catarrhalis in patients of lower respiratory tract infection with underlying risk factors. Int J Adv Med 2017; 4; 442-445
  • Sushitha TS, Suseela KV . A Prospective Cohort Study on the Infections Associated with Indwelling Medical devices in a Tertiary Care Hospital in South India. JMSCR: 2018; 06 ; 87-94
  • Suseela KV, Varma R. Campylobacter–induced diarrhoea in an infant. case report. J Acad ClinMicrobiol; 2018 ; 20; 102 - 104
  • Deepa MK, Suseela KV. Utility of Anti-Helicobacter pylori IgG estimation in the Diagnosis of Acid peptic Diseases: A comparison of Serum IgG Level with Rapid Urease Test. IJCMR: 2019; 6(8):H1-H3
  • Chacko AA, Subbiah R, Varghese TC, Suseela KV. Mycoplasma pneumonia among children with acute respiratory infection. Int J Contemp Pediatr 2021; 8:550-3.
  • Suseela KV, Khan F , Kottoor S J. An unusual case of neutrocytic, culture-negative meningitis in an immunocompetent adult. JRSM, Open 2021;12 (5):20542704211009354. doi: 10.1177/20542704211009354
  • Aiswariya A, Pavani K, Rajendra BS. Bacteriology of diabetic foot infections and their antibacterial susceptibility. Int J Res Med Sci 2018;6:3276-80.
  • Aiswariya A, Suseela K V, Subi D. Prevalence of Moraxella catarrhalis in patients of lower respiratory tract infections with underlying risk factors. Int J Adv Med 2017;4:442-5.
  • Allen S, Suresh S, Aiswarya A. A rare case of Burkholderia pseudomallei presenting as brain abscess , EJMHS 2021;3:24-27.
  • Subi Das, Kalpana George, Girija Kalarikkal Rajagopal. Prevalence and factors associated with the nasal colonization of Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus among patients in a tertiary care hospital: Indian Journal of Mic
  • Subi Das, Prithi Nair Kannambra. Minimum Inhibitory concentration of Vancomycin against Methicillin Resistant Staphylococcus aureus strains isolated from a tertiary care hospital: Journal of Clinical and diagnostic research.2020 Apr, Vol-14(4):DC01- Dc05
  • Geethalakshmi, KA Jasmine, Anu P John, Priya Prathap. .Effectiveness of Sabouraud's dextrose agar and dermatophyte test medium in detection of candidiasis and dermatophytosis in superficial skin lesion,Journal of Clinical and Diagnostic Research. 2021 Aug, Vol-15(8): DC11-DC15
  • Babu N, Saikumar C, Chalissery JR,Kumar H.Extracellular enzymatic activity of Candida species isolated from patients with head and neck cancer undergoing radiation therapy and their correlation with mucositis-A cross-sectional study. Journal of Clinical and Diagnostic Research(Accepted).2023.
  • Ramya K A, Anu S. S, Harish Kumar K. S, Shinu K, Neethu B. Antibacterial Activity of Silver, Gold and Amoxicillin-Silver Nanoparticles against Methicillin Resistant Staphylococcus aureus - an invitro Study . Int J Cur Res Rev. 15(10), May, 2023, 01-04, https://doi.org/10.31782/IJCRR.2023.151001
  • Babu N, Saikumar C, Chalissery JR, Kumar H.Extracellular Enzymatic Activity of Candida Species Isolated from Patients with Head and Neck Cancer Undergoing Radiation Therapy and Its Correlation with Mucositis: A Cross-Sectional Study J Clin of Diagn Res.2023; 17(9):DC01-DC05. https://www.doi.org/10.7860/JCDR/2023/65554/18373.
  • Babu N, Saikumar C, Chalissery JR.A Study on Subsite-specific Prevalence of Candidiasis in Head and Neck Cancer Patients and its Antifungal Susceptibility Pattern: A Cross-sectional StudyJ Clin of Diagn Res.2023; 17(10):DC09-DC13. https://www.doi.org/10.7860/JCDR/2023/66418/18509.
  • Babu N, Saikumar C, Chalissery JR.Adherence of Candida species isolated from patients with head and neck cancer  presented for radiation therapy and its correlation with hemogram.Journal of pure and applied Microbiology 2023(Accepted)
  • Neethu B, Chitralekha S, Jomon Raphael C. Mechanisms of Azole Resistance in Candida- A Narrative Review . Int J Cur Res Rev. 15(20), November, 2023, 05-12, https://doi.org/10.31782/IJCRR.2023.152002.
  • Sasikumari,O.,George,A. M., & Sarada Devi, K.L. (2021). Extended Spectrum Beta Lactamase (ESBL) Producing Neisseria meningitidis- Isolated from a Case of Pneumonia. European Journal of Medical and Health Sciences, 3(3), 75-77
  • Francis R, Rangaiah A, Rangappa KG, Venugopal SJ.Direct Detection of Extended Spectrum β-Lactamases from Positive Blood Cultures by using Aztreonam and Clavulanate.J Clin of Diagn Res.2021; 15(6).
  • Renji Francis N, Jyoti S Kabbin. 2023. “Microbiological profile of keratomycosis in a tertiary care hospital. A retrospective study.International Journal of Current Research, 15, (01), 23351-23353.

    https://doi.org/10.24941/ijcr.44670.01.2023

  • K.G Chithira, Reena John, Prithi Nair K, Seroprevalence of Hepatitis E in healthy adults attending a tertiaryvcare centre in central Kerala. Journal of the Academy of ClinicalbMicrobiologists. 2019 volume 21 issue 2 page 80-84.
  • Heera Hassan, Reena John, Prithi Nair K, M.A. Andrews. Detection and serotyping of Dengue virus in a tertiary care centre in Thrissur, Kerala- a Cross sectional study. WHO Dengue bulletin Volume 40 December 2018
  • Anjali Nair Vinayakumar, Reena John. Seroprevalence of HepatitisC infection in patients undergoing hemodialysis in a tertiary care centre- Journal of Patient safety and infection control volume 8 issue 2 May- August 2020
  • Reena John, Dagny Hari, Prithi Nair K. Urinary tract infections- Bacteriologic profile and antibiotic sensitivity pattern in a tertiary care hospital. Indian Journal Of Applied Research Volume 8Issue 1January 2018
  • Reena John, Anupama Sethumadhavan, Prithi Nair K. Changing trends in Antibiotic Resistance Profile of Salmonella enteritica species in a Tertiary Care Hospital in Central Kerala. JMSCR Volume 5 Issue 10
  • Renjini Joseph, Reena John, Prithi Nair K . Infected Orthopaedic Implants and Infectious Agents: MRSA frequency and Infection Patterns, In a tertiary Care Centre in Central Kerala.
  • Clinical Characterization and Genomic Analysis of Samples from COVID-19 Breakthrough Infections during the Second Wave among the Various States of India. Nivedita Gupta 1,Harmanmeet Kaur 1,Pragya Dhruv Yadav 2,*ORCID,Labanya Mukhopadhyay 1ORCID,Rima R. Sahay 2ORCID,Abhinendra Kumar 2,Dimpal A. Nyayanit 2,Anita M. Shete 2,Savita Patil 2,Triparna Majumdar 2,Salaj Rana 1,Swati Gupta 1,Jitendra Narayan 1ORCID,Neetu Vijay 1,Pradip Barde 3ORCID,Gita Nataraj 4,Amrutha Kumari B. 5,Manasa P. Kumari 5,Debasis Biswas 6,Jyoti Iravane 7,Sharmila Raut 8,Shanta Dutta 9ORCID,Sulochana Devi 10,Purnima Barua 11,Piyali Gupta 12,Biswa Borkakoty 13,Deepjyoti Kalita 14,Kanwardeep Dhingra 15,Bashir Fomda 16,Yash Joshi 2,Kapil Goyal 17, Reena John 18, Ashok Munivenkatappa . Viruses 2021, 13(9), 1782; https://doi.org/10.3390/v13091782 Academic Editors: Burtram C. Fielding and Georgia Schäfer. 18:Viral Research and Diagnostic Laboratory, Government Medical College, Thrissur 680596, India ((This article belongs to the Special Issue Coronaviruses Research in BRICS Countries)